716 Rbc Stroma Activation
نویسنده
چکیده
The syndrome of disseminated intravascular coagulation (DIC) 1 is a recognized complication of acute hemolytic episodes in both man (1-3) and laboratory animals (4, 5) and has been induced experimentally in monkeys and dogs by infusion of autologous red blood cell stroma (6, 7). The mechanism of stromainduced D I C may be analogous to the demonstrable procoagulant activity of red cell membrane phospholipids in vitro. However, an additional or alternative chain of events involving activation of the complement system is suggested by the well-studied model of DIC , the Sanarelli-Shwartzman reaction, produced by two appropriately spaced intravenous injections of bacterial endotoxin. The recent work of Fong and Good (8) and Margaret ten (9) indicate a requirement for platelets and an intact complement system for endotoxin-induced DIC. Complement activation by endotoxin proceeds primarily, if not entirely, via the recently described alternate pathway (10). In view of the ultrastructural similarity between endotoxin and red cell stroma (i.e., bilaminar membrane [11]) and their ability to produce DIC, we have initiated studies to determine whether red cell stroma is also capable of activating the alternate complement pa thway and to a t tempt to relate this function to the sequence of events leading to DIC.
منابع مشابه
Activation of the Alternate Complement Pathway by Autologous Red Cell Stroma
The present study demonstrates the ability of human autologous RBC stroma to activate the alternate complement pathway (C3-Activator system, properdin system), as evidenced by the generation of C3-Activator (C3A) from C3-Proactivator (C3PA) when RBC ghosts and sonicated ghosts are incubated with autologous serum. Intact RBC's, hemoglobin, and concentrated platelet stroma, on the other hand, are...
متن کاملSome Physical and Immunological Reactions of RBC 'Juice' and Stroma
The following experiments with rbc juice and stroma were undertaken to answer two questions : 1. Is the Hb in solution inert with an antiserum-protein serum and a blood-group specific serum ? 2. Can anti-rbc serum (such as antiRh serum, and anti-M&N fluids) be prepared from debris instead of whole cells which may not be compatible with the blood of the animal injected ? The first question is of...
متن کاملبررسی مقایسه تأثیرات نانوذرات نقره بر میزان تغییرات گلبولهای خونی در موشهای صحرایی نر
Introduction: Nanosilver particles are one of the functional grounds in nanotechnology field. These nanoparticles may produce free radicals and destruct different cells. In this study, Nanosilver toxic effects on RBC and WBC numbers in male Rats were studied. Thus, male rats were treated with nanosilver and RBC and WBC were detected. Methods: In this study, RBC was detected in male Wistar rats...
متن کاملModerate Exercise Promotes Human RBC-NOS Activity, NO Production and Deformability through Akt Kinase Pathway
BACKGROUND Nitric oxide (NO) produced by nitric oxide synthase (NOS) in human red blood cells (RBCs) was shown to depend on shear stress and to exhibit important biological functions, such as inhibition of platelet activation. In the present study we hypothesized that exercise-induced shear stress stimulates RBC-NOS activation pathways, NO signaling, and deformability of human RBCs. METHODS/F...
متن کاملIncrease in Red Blood Cell-Nitric Oxide Synthase Dependent Nitric Oxide Production during Red Blood Cell Aging in Health and Disease: A Study on Age Dependent Changes of Rheologic and Enzymatic Properties in Red Blood Cells
AIM To investigate RBC-NOS dependent NO signaling during in vivo RBC aging in health and disease. METHOD RBC from fifteen healthy volunteers (HC) and four patients with type 2 diabetes mellitus (DM) were separated in seven subpopulations by Percoll density gradient centrifugation. RESULTS The proportion of old RBC was significantly higher in DM compared to HC. In both groups, in vivo aging ...
متن کامل